Direct PCR from solid tissues without DNA extraction
نویسندگان
چکیده
منابع مشابه
Direct PCR from solid tissues without DNA extraction.
on the use of formamide and reduced incubation temperatures. Here we describe a modification of FoLT PCR which allows direct amplification from solid tissue samples without the need for any DNA purification steps. Two oligonucleotide primer combinations directed against the murine I82 microglobulin gene were used in this study. Using a standard PCR protocol it was not possible to amplify the ap...
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Typically DNA used in PCR assays is usually extracted according to die phenol-chloroform method (1) or by an alternative 'saltingout' rapid purification (2). Moreover partially purified DNA obtained with rapid procedures have been reported to be suitable for PCR amplification (3). We describe here a more simple and efficient method to amplify DNA directly from whole blood samples without any pu...
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DNA extraction before amplification is considered an essential step for quantification of viral DNA using real-time PCR (qPCR). However, this can directly affect the final measurements due to variable DNA yields and removal of inhibitors, which leads to increased inter-laboratory variability of qPCR measurements and reduced agreement on viral loads. Digital PCR (dPCR) might be an advantageous m...
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Molecular diagnostics are performed by using DNA from different body tissues. However, it is necessary to obtain genomic DNA of good quality. Due to the impossibility of collecting blood from slaughtered animals, DNA extraction from solid tissues is necessary. The objective of this study was to describe a protocol of DNA extraction from swine skin, adipose, brain, liver, kidney and muscle tissu...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1993
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/21.19.4656